Cloning, Bioinformatics Analysis and Physiological Function of the Pine Wood Nematode Bxadh2 Gene

In previous research, the pine wood nematode Bxadh2 gene significantly increased its expression in seedlings inoculated with endophytic Bacillus cereus GD2 and nematode. To explore function, we cloned analyzed biological information, preliminarily studied physiological function through RNA interference. We found that gene’s full CDS length is 1269 bp, which encodes 422 amino acids, presents a relatively stable hydrophobic protein. The protein encoded by has no signal peptide or transmembrane structure, it an intracellular does not participate movement. RNAi interference results showed when nematode’s was suppressed, survival rate fecundity decreased, indicating of related to growth development nematodes.